Volume 7 | Issue -2
Volume 7 | Issue -2
Volume 7 | Issue -2
Volume 7 | Issue -2
Volume 7 | Issue -2
Introduction: Luffa acutangula belongs to family cucurbitaceae, called as ridge gourd, angled gourd or angled luffa with greater health benefits. The present study was aimed to characterize the phytochemical profile for various secondary metabolites using phytochemical screening, FTIR analysis and HPTLC fingerprinting of ethanolic fruits and leaves extract of Luffa acutangula. Methods: The fresh fruits and leaves were extracted using Soxhlet apparatus, extract was further used for analysis of Phytochemical compounds by chemical reaction. Then identified by Fourier Transform Infrared Spectrophotometer (FTIR) profiling and High-Performance Thin Layer Chromatography (HPTLC) analysis. Results: Quantitative analysis revealed that ethanolic fruit and leaves extract consists of alkaloids, glycosides, saponins, tannins, flavonoids, terpenoids and phenols. The FT-IR spectrum of ethanolic fruit and leaves extract of Luffa acutangula demonstrated the presence of C-H alkanes, O-H Hydroxyl, C=O carboxylic acid, ether and glycosidic bond. The HPTLC densitometric analysis of the ethanolic fruit and leaves extract of Luffa acutangula was carried out using CAMAG HPTLC system and chromatograms were scanned at 254 nm representing several peaks. HPTLC data of extract shows ten to fifteen different peaks confirming that bioactive compounds are present. HPTLC finger printing of ethanolic fruit extract revealed 11 peaks with Rf values in the range of 0.05 to 0.92; extract of leaves showed 14 peaks with Rf values in the range of 0.05 to 0.99. Conclusion: The study concluded that the fruit and leaves extract of Luffa acutangula contain a rich variety of phytochemicals. FTIR and HPTLC fingerprint analysis of extracts of Luffa acutangula can be done as a characteristic tool for the correct identification of the plant.