Current research aimed to develop an edible herbal tooth gel for the treatment of periodontitis using Vaccinium macrocarpon fruit extract. The formulation was prepared using conventional techniques and 23 full factorial designs. The final formulation had a reddish-pink colour, smooth appearance, thick consistency, and high viscosity. The gel was tested for its various in vitro, in-vivo, and ex-vivo studies such as appearance, viscosity, spread ability, pH, foaming activity, fineness, abrasiveness, stickiness, and grittiness, homogeneity, cleaning ability, antibacterial activity and normal flora. The gel was found to have no negative effects on normal gastrointestinal flora (E. coli). The study concluded that the herbal edible tooth gel is natural, free from SLS, probiotic-loaded, and safe in the gastrointestinal tract if swallowed unintentionally. As per the outcome of the study, it was detected effective for dental care and periodontitis.