Amla, Neem, Mulethi and Tulsi are known to have medicinal properties. These herbs are quite beneficial for treating many diseases. Amla, Neem, Mulethi and Tulsi have been used by individuals to cure respiratory tract infections, gastrointestinal conditions, cough, cold, bronchitis, and asthma. It has also been observed that the immunological condition has improved. These historic uses of plant medications include treating heart disease, hepatitis, fungal infections, malaria, psoriasis, ulcers, and sores. In the present research polyherbal gel formulation of Amla, Neem, Mulethi and Tulsi extracts for Mouth ulcer are prepared and evaluated. Drug compatibility with specific excipients used in the formulation was tested using densitometry TLC to determine the drug concentration. The almost similar Rf values that were obtained indicated that Amla, Neem, Mulethiand Tulsi were compatible with the excipients utilized in the gel formulation. Percentage drug release study, thus conducted, revealed that about 81.110% Gallic acid (Amla), 82.006% Azadirachtine (Neem), 83.556% Glycyrrhizic acid (Mulethi) and 81.744 % Ursolic acid (Tulsi) were released from the prepared polyherbal gel. Ex-vivo drug release revealed that about 83.301% Gallic acid (Amla), 86.594% Azadirachtine (Neem), 86.989% Glycyrrhizic acid (Mulethi) and 83.413% Ursolic acid (Tulsi) were released from the prepared polyherbal gel. In conclusion, polyherbal gel may be clinically effective in treating canker sores. It may also be worth exploring further for other ulcerative disorders with topical or surface origins.