Background: School students' lives are seen to be seriously threatened by unintentional injury. First aid education through game-based learning may be appropriate for schoolchildren's developmental stage and help them advance their knowledge and abilities. Aim: to evaluate the effect of a game-based training program on school students' knowledge and practices about first aid. Method: A quasi experimental study with a one-group pre-post-test design including 60 preparatory school students in two governmental preparatory schools, representing urban and rural areas in Sherbin Center. Tools: This study used three tools for collecting data about school students’ socio-demographic and academic attributes, knowledge, and practice toward first aid. The results: 93.3% of school students had an unsatisfactory total score level of knowledge in the pretest, which changed to 93.3%, and 85.0% had a satisfactory total score level of knowledge in the immediate test and after one-month post-test, respectively. Related to practice, 5.0% of school students had adequate total score levels in the pretest that changed to 100% in the immediate test and after one month of post-test. Conclusion: There was an improvement in school students' knowledge and practice levels immediately post-implementation and one month later compared to pre-implementation of game-based training. Recommendations: It is recommended to install game-based programs to empower schools' health education programs that prioritize the most prevalent injuries and first-aid scenarios.