ISSN : 2663-2187

Phytochemical and Pharmacological Evaluation of Antioxidant and Antidiabetic Potential of Wedelia calandulaceae Leaves Extract: Focus on Alpha-Amylase and Alpha-Glucosidase Inhibition

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Afreen Shahid, Aisha Kamal, Kunal, Manpreet Kaur, Akhilesh Maurya, Dinesh Vishwakarma, Shashi Kant, Shweta Hitesh Shahare
» doi: 10.33472/AFJBS.6.6.2024.7715-7729

Abstract

The present study was designed to pharmacologically evaluate the therapeutic potential of Wedelia calandulaceae Less. non-Rich. (Family: Asteraceae). Antioxidant and antidiabetic potentials were investigated. The phytochemical screening of the methanolic extract of Wedelia calandulaceae (WCME-L) revealed a diverse array of bioactive compounds, including high concentrations of flavonoids and phenols, and significant amounts of saponins, alkaloids, phytosterols, tannins, terpenoids, and glycosides. Despite the measurable phenolic content of 8.53 mg GAE/g extract, the study found no direct correlation with antioxidant activity, suggesting other factors at play. The thiocyanate method demonstrated potent antioxidant properties at 250 µg/ml, with the extract showing higher activity than α-Tocopherol but lower than BHA. Reducing power assays confirmed the extract's strong antioxidant capacity, surpassing α-Tocopherol and being second to BHA. In DPPH radical scavenging and superoxide anion scavenging activities, the extract exhibited significant efficacy, though slightly less potent than BHA and ascorbic acid, respectively. The extract also displayed dose-dependent alpha-amylase and alpha-glucosidase inhibition, indicating potential for managing blood sugar levels in diabetes. These findings suggest that WCME-L is a potent natural antioxidant with promising therapeutic applications, particularly in managing oxidative stress and metabolic disorders.

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