One of the most common and significant opportunistic fungal infections, candidiasis, is brought on by Candida species such as Candida albicans, Candida glabrata, and Candida krusei. Typically, amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole, and echinocandins are used to treat suspected Candida infections. Careful selection of an antifungal medication is required for treatment due to the potential risk of azole-resistant Candida isolate strains emerging. Medicinal herbs have anti-infective properties against bacteria, viruses, and fungi. This study's goal is to assess the in vitro effectiveness of popular Indian medicinal plants, including neem, tulsi, and aloe vera, against isolates of Candida sp. derived from clinical samples. The clinical samples gathered include oral swabs, vaginal swabs, nail scrapings, and skin scrapings. The samples were cultivated in the lab, where Candida sp. was isolated and identified. Testing for antifungal disk diffusion susceptibility was done. The medicinal herbs' aqueous and alcoholic extracts were made, and the Minimum Inhibitory Concentration (MIC) against Candida sp. was calculated. 35 out of 180 Candida isolates were discovered to be drug-resistant, which means that 19% of all isolates were resistant. All of the resistant isolates were just C. tropicalis. Finally, it can be seen that alcoholic aloe vera leaf extracts have greater anticandidal action than aqueous extracts. Neem leaf alcohol extract has a comparable impact on Candida species.