In recent times, a series of 10- [2-substitutedimino-4-substituted amino)-1,3,5-dithiazino] Chlorophenothiazines was synthesized by the interaction with substituted isocyanodichloride having 50% acetone-ethanol solvent by conventional method. This method has broad applications due to the efficacy of this approach makes it suitable for accelerating a variety of organic processes, resulting in increased yield, improved selectivity, and reduced production of by-products. As a result, this approach provides a simpler process for extracting the desired products, which are produced in a relatively pure state. Synthesized compounds are verified by elemental analysis, chemical characterization and spectral analysis like IR, NMR and Mass. All synthesized compounds (4a-d) showed moderate-to-significant anti-bacterial and anti-fungal activity. Compound 4d showed moderate activity against breast carcinoma (MCF-7) cell lines with IC50 values of 49.52 µM.