ISSN : 2663-2187

VIABILITY STUDY OF TAPAK LIMAN (Elephantopus scaber Linn) LEAVES TOWARD RAW 264.7 CELLS

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Mega Yulia , Yufri Aldi , Hansen Nasif , Fatma Sri Wahyuni
» doi: 10.48047/AFJBS.6.7.2024.3226-3232

Abstract

Tapak liman (Elephantopus scaber Linn) is a plant that is easily found in Indonesia which is traditionally used for various treatments. The chemical constituents of the E. scaber plant are sesquiterpenes, triterpenoids, steroids, flavonoids, tannins, and saponins. The aim of the stydy is to determine the safety of using this plant, by looking the effect of this plant's leaf extract on cell growth by carrying out a viability study. A viability study was carried out on raw 264.7 cells. This study was design as laboratory experiment. E.scaber was extracted using cold maceration method with ethanol, then ethanol was evaporated with rotary evaporator. Before cell viability study, cells were cultivated in 96 well plates and providing ethanol extract from E. scaber leaves at concentrations of 500, 100, 50, 25, 12.5, 6.25, and 1 µg/ml. Then cells were cultured at 37oC with 5% CO2 for 48 hours. After 48 hours the cells added with 100 µl of MTT solution with a concentration of 0.5 mg/ml in PBS for about 4 hours. The resulting crystals of formazan then dissolved in 100 μl DMSO, and their absorbance was determined using a microplate reader. Cell viability was observed in the ethanol extract of E. scaber leaves, each of them at concentrations of 500, 100, 50, 25, 12.5, 6.25, and 1 µg/ml were 15.35%, 94.29%, 115.30%, 121.36%, 120.23%, 111.74%, 102.29%. From the study results it can be seen that the cell viability of E.scaber ethanol extract for concentrations 1-100 µg/ml gives a cell viability percentage above 80%. From this study, it can be concluded that E. scaber leaf extract was not harmful to raw cells and and shows potential for further study into plant's medicinal properties because the extract able to maintain cell viability above 80% at concentrations ranging from 1 to 100 μg/ml.

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